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nikon eclipse ni e microscope  (Nikon)


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    Structured Review

    Nikon nikon eclipse ni e microscope
    Nikon Eclipse Ni E Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 63936 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microscope+objective/Objectives/pmc13672656-106-8-8
    Average 99 stars, based on 63936 article reviews
    nikon eclipse ni e microscope - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    other:

    Article Title: Bubbles nucleation in supersaturated emulsion drops.
    Article Snippet: Stirring was stopped, and changes in volume were recorded with an accuracy of ≈ 0.3 mL and a typical reproducibility of ± 0.50 mL. (5) In independent repetitions of the experiments, we took samples during the final stages of stirring for microscope observation between 3 glass slides via ×5 LD objective (Nikon E Plan).

    Article Title: The estrous cycle modulates hippocampal spine dynamics, dendritic processing, and spatial coding.
    Article Snippet: For imaging, we used a 16×/0.8 NA microscope objective (Nikon) to collect 760 × 760 pixel frames with field sizes ranging from 829× 829 μm (1x zoom) to 51.8× 51.8 μm (16x zoom).

    Article Title: Bacteria Optimize Tumble Bias to Strategically Navigate Surface Constraints.
    Article Snippet: Both beams were tightly focused by a 60×, waterimmersion (1.2 NA) microscope objective (Nikon) beneath the sample chamber to generate two separated optical traps.

    Article Title: Multimodal Investigation of Angiogenesis and Its Prevention by Small Compounds in a Zebrafish Cancer Model.
    Article Snippet: The laser beam was focused onto the sample through a 4x Nikon microscope objective with 0.1 numerical aperture (NA) and a working distance of 30 mm.

    Article Title: Light-responsive polypeptides and methods of use thereof
    Article Snippet: Because of the piezo movement, the constellation of optogenetic targets will be axially offset during each frame acquisition, requiring a compensating quadratic phase offset to the SLM phase masks for each axial position. (D) The physiological response, as characterized by the percentage change in GCaMP fluorescence, when using a 15 μm diameter spiral for optogenetic photostimulation (9-spirals, 0.63 ms duration, as used in behavior experiments; n=10 neurons in vivo, mean response across 5 trials per stimulation location—the spiral was intentionally shifted from center of each cell by the defined lateral offset—randomized and interleaved, normalized to maximum for each cell, fit with a single gaussian function, error bars are s.e.m.). (E) Empirical, axial optical PSF measurements of both the optical imaging beam (FWHM=9.6 μm, NAest=0.64) and an optogenetic stimulation beam (FWHM=21.4 μm, NAest=0.48) using the Nikon 16×/0.8 NA microscope objective in the MultiSLM microscope.

    Tungsten:

    Article Title: Boosting Atomic‐Scale Light‐Matter Interactions With Plasmonic Structures Thinned to the Nanometer Limit
    Article Snippet: Enhancing inherently-weak light-matter interactions in two-dimensional (2D) photoactive materials is critical for fundamental research and developing next-generation nanophotonic and quantum optical devices.. Conventional approaches utilizing plasmonic nanostructures of at least tens of nanometers in thickness, however, suffer from significant mode and physical size mismatch with the atomically thin 2D materials, limiting both the enhancement factor and device miniaturization.. Here, we overcome these constraints by downscaling the thickness of plasmonic nanostructures to the nanometer limit.

    Microscopy:

    Article Title: Boosting Atomic‐Scale Light‐Matter Interactions With Plasmonic Structures Thinned to the Nanometer Limit
    Article Snippet: Enhancing inherently-weak light-matter interactions in two-dimensional (2D) photoactive materials is critical for fundamental research and developing next-generation nanophotonic and quantum optical devices.. Conventional approaches utilizing plasmonic nanostructures of at least tens of nanometers in thickness, however, suffer from significant mode and physical size mismatch with the atomically thin 2D materials, limiting both the enhancement factor and device miniaturization.. Here, we overcome these constraints by downscaling the thickness of plasmonic nanostructures to the nanometer limit.

    Article Title: Exciton diffusion beyond 2 μm enabled by maximum symmetry in two-dimensional perovskites
    Article Snippet: High crystallographic symmetry is a key feature of many inorganic semiconductors and underpins their remarkable physical properties.. By contrast, hybrid (organic and inorganic) crystals such as two-dimensional metal halide perovskites exhibit much lower crystal symmetry due to in-plane or out-of-plane octahedral distortions.. Although they exhibit efficient photoinduced emission at room temperature, limited control over charge carrier transport remains a major challenge for optoelectronic applications.

    Article Title: Non-Destructive and Real-Time Virtual Staining of Spermatozoa via Dark-Field Microscopy.
    Article Snippet: Sperm morphology serves as a crucial indicator of fertilization potential; however, the fixation and staining required for its assessment irreversibly result in irreversible damage to sperm.. Here, an improved Generative Adversarial Network (GAN) virtual staining model based on darkfield microscopy enables realtime conversion of labelfree semen smears into highcontrast Papanicolaouequivalent stained images.. The experimental results demonstrated that our network completed virtual staining in ~0.047 s for a 2048 × 2048 image.

    Imaging:

    Article Title: Non-Destructive and Real-Time Virtual Staining of Spermatozoa via Dark-Field Microscopy.
    Article Snippet: Sperm morphology serves as a crucial indicator of fertilization potential; however, the fixation and staining required for its assessment irreversibly result in irreversible damage to sperm.. Here, an improved Generative Adversarial Network (GAN) virtual staining model based on darkfield microscopy enables realtime conversion of labelfree semen smears into highcontrast Papanicolaouequivalent stained images.. The experimental results demonstrated that our network completed virtual staining in ~0.047 s for a 2048 × 2048 image.



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    In vitro properties of Im@NOnanoPL nanovaccine. (A) Flow cytometric analysis of DC2.4 cells co‐incubated with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5 after different durations, respectively. (B) Percentage of Cy5 + DC2.4 cells after incubation with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5. (C) Confocal microscopic images of DC2.4 cells after incubating with Im@NOnanoPL‐Cy5 for 2 h and 6 h. The lysosomes stained with Lysotracker Green (green) and amide‐coupled Cy5 labeling for stable cell membrane vesicles surface modification, Scale bar: 10 µm. (D) Evaluation of MHC‐I levels in BMDCs after 12 and 24 h incubation with Im@NOnanoPL and other formulations (NOC‐18: 100 µM), as detected by flow cytometry ( n = 4). (I: PBS, II: m@nanoPL, III: Im@nanoPL, IV: m@NOnanoPL, V: Im@NOnanoPL). (E) Western blot analysis of cGAS‐STING pathway‐related proteins expression in BMDCs treated by Im@NOnanoPL and other formulations (NOC‐18: 100 µM) in 12 h (G1: PBS, G2: m@nanoPL, G3: Im@nanoPL, G4: m@NOnanoPL, G5: Im@NOnanoPL). (F) Experimental design for T cell activation and cytokine release analysis. BMDCs were treated with different formulations for 12 h, followed by co‐culture with CD3 + T cells at <t>a</t> <t>1:10</t> ratio (BMDCs: T cells). The CD3 + T cells were isolated from splenocytes of MC38 tumor‐bearing mice. (G) Percentages of CD69 + or CD25 + in CD3 + CD8 + T cells after 24 h co‐culture, as detected using flow cytometry ( n = 4). (H) Cytokines released by T cells and DCs from different groups after 24 h co‐culture, including IL‐6, IL‐1β, TNF‐α, IFN‐β. Results are presented as mean ± SD ( n = 4). Statistical analysis was performed using the one‐way ANOVA with Tukey's multiple comparisons test.
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    Image Search Results


    In vitro properties of Im@NOnanoPL nanovaccine. (A) Flow cytometric analysis of DC2.4 cells co‐incubated with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5 after different durations, respectively. (B) Percentage of Cy5 + DC2.4 cells after incubation with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5. (C) Confocal microscopic images of DC2.4 cells after incubating with Im@NOnanoPL‐Cy5 for 2 h and 6 h. The lysosomes stained with Lysotracker Green (green) and amide‐coupled Cy5 labeling for stable cell membrane vesicles surface modification, Scale bar: 10 µm. (D) Evaluation of MHC‐I levels in BMDCs after 12 and 24 h incubation with Im@NOnanoPL and other formulations (NOC‐18: 100 µM), as detected by flow cytometry ( n = 4). (I: PBS, II: m@nanoPL, III: Im@nanoPL, IV: m@NOnanoPL, V: Im@NOnanoPL). (E) Western blot analysis of cGAS‐STING pathway‐related proteins expression in BMDCs treated by Im@NOnanoPL and other formulations (NOC‐18: 100 µM) in 12 h (G1: PBS, G2: m@nanoPL, G3: Im@nanoPL, G4: m@NOnanoPL, G5: Im@NOnanoPL). (F) Experimental design for T cell activation and cytokine release analysis. BMDCs were treated with different formulations for 12 h, followed by co‐culture with CD3 + T cells at a 1:10 ratio (BMDCs: T cells). The CD3 + T cells were isolated from splenocytes of MC38 tumor‐bearing mice. (G) Percentages of CD69 + or CD25 + in CD3 + CD8 + T cells after 24 h co‐culture, as detected using flow cytometry ( n = 4). (H) Cytokines released by T cells and DCs from different groups after 24 h co‐culture, including IL‐6, IL‐1β, TNF‐α, IFN‐β. Results are presented as mean ± SD ( n = 4). Statistical analysis was performed using the one‐way ANOVA with Tukey's multiple comparisons test.

    Journal: Exploration

    Article Title: A Nitric Oxide–Based STING Agonist Nanoadjuvant With ICD‐Derived Membrane Antigens Unleashes Robust Antitumor Immunity

    doi: 10.1002/exp2.70238

    Figure Lengend Snippet: In vitro properties of Im@NOnanoPL nanovaccine. (A) Flow cytometric analysis of DC2.4 cells co‐incubated with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5 after different durations, respectively. (B) Percentage of Cy5 + DC2.4 cells after incubation with Im@NOnanoPL‐Cy5 and m@NOnanoPL‐Cy5. (C) Confocal microscopic images of DC2.4 cells after incubating with Im@NOnanoPL‐Cy5 for 2 h and 6 h. The lysosomes stained with Lysotracker Green (green) and amide‐coupled Cy5 labeling for stable cell membrane vesicles surface modification, Scale bar: 10 µm. (D) Evaluation of MHC‐I levels in BMDCs after 12 and 24 h incubation with Im@NOnanoPL and other formulations (NOC‐18: 100 µM), as detected by flow cytometry ( n = 4). (I: PBS, II: m@nanoPL, III: Im@nanoPL, IV: m@NOnanoPL, V: Im@NOnanoPL). (E) Western blot analysis of cGAS‐STING pathway‐related proteins expression in BMDCs treated by Im@NOnanoPL and other formulations (NOC‐18: 100 µM) in 12 h (G1: PBS, G2: m@nanoPL, G3: Im@nanoPL, G4: m@NOnanoPL, G5: Im@NOnanoPL). (F) Experimental design for T cell activation and cytokine release analysis. BMDCs were treated with different formulations for 12 h, followed by co‐culture with CD3 + T cells at a 1:10 ratio (BMDCs: T cells). The CD3 + T cells were isolated from splenocytes of MC38 tumor‐bearing mice. (G) Percentages of CD69 + or CD25 + in CD3 + CD8 + T cells after 24 h co‐culture, as detected using flow cytometry ( n = 4). (H) Cytokines released by T cells and DCs from different groups after 24 h co‐culture, including IL‐6, IL‐1β, TNF‐α, IFN‐β. Results are presented as mean ± SD ( n = 4). Statistical analysis was performed using the one‐way ANOVA with Tukey's multiple comparisons test.

    Article Snippet: Imaging was performed on a Nikon A1R HD25 confocal microscope using a 60× oil objective.

    Techniques: In Vitro, Analysis, Incubation, Confocal, Staining, Labeling, Stable Transfection, Membrane, Modification, Flow Cytometry, Western Blot, Expressing, Activation Assay, Co-culture Assay, Isolation